Comparison of phenotypic methods for penicillinase detection in Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Evaluation of Five Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
BackgroundandObjectives:RapidandaccuratedetectionofmethicillinresistantStaphylococcus aureus (MRSA) is an important role of clinical microbiology laboratories to avoid treatment failure. The aim of this study was to compare conventional methods against the E-test minimum inhibitory concentration (MIC) method to determine the best phenotypic method. Materials a...
متن کاملEvaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...
متن کاملPerformance of phenotypic tests for detection of penicillinase in Staphylococcus aureus isolates from Australia.
Recent studies have shown that chromogenic cephalosporin tests are inferior to disc zone edge tests in detecting penicillinase in Staphylococcus aureus isolates, resulting in a change to CLSI and EUCAST guidelines in 2012. We sought to confirm these findings using Australian isolates and compare the performance of the CLSI and EUCAST methods, which use different disc strengths, penicillin at 10...
متن کامل[Phenotypic methods for detection of methicillin-resistant Staphylococcus aureus].
BACKGROUND Cefoxitin is a potent inducer of the mecA gene. It is currently as a screening recommended method for presumptive identification of isolates of methicillin resistant Staphylococcus aureus (MRSA). The aim of the study was to compare the sensitivity and specificity of the cefoxitin disc diffusion (30 μg) to oxacillin agar screening from detection of the mecA gene by PCR. METHODS Thre...
متن کاملComparison of phenotypic versus genotypic methods in the detection of methicillin resistance in Staphylococcus aureus.
BACKGROUND & OBJECTIVE Conventional methods to detect methicillin resistance in Staphylococcus aureus are inadequate as expression of resistance is subject to environmental and conditional expression of PBP2a antigen. The objective of the present study was to determine methicillin resistance in S. aureus by conventional susceptibility (oxacillin disc diffusion and oxacillin MIC) and molecular m...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2008
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2008.01997.x